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KMID : 0380020060210060479
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 6 p.479 ~ p.483
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Optimization of Medium Components for Cell Mass Production of Saccharomyces cerevisiae JUL3 using Response Surface Methodology
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Kim Young-Hwan
Lee Jong-Ho
Kang Seong-Woo
Chang Hyo-Ihl
Yun Cheol-Won
Paik Hyun-Dong
Kang Chang-Won
Kim Seung-Wook
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Abstract
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[ ], one of the cell wall components, is most plentiful polysaccharides in cell wall and has several advantages in immune system. In yeast is mainly contained in the yeast cell wall, and thus it is important to produce high levels of cell mass for the mass production of yeast . The best carbon and nitrogen sources on cell mass production were high fructose syrup and yeast extract. Response surface methodology (RSM) was very potential tool for the optimization of process factor and medium component. It was applied to estimate the effects of medium components on the production of cell mass. Optimal concentrations of high fructose syrup and yeast extract by response surface methodology were 8.0% (v/v) and 5.2% (w/v), respectively and the cell mass predicted was at 20 h of cultivation.
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KEYWORD
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β-Glucan, Saccharomyces cerevisiae JUL3, high fructose syrup, response surface methodology
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